Mediterranean Journal of Hematology and Infectious Diseases

Background: Worldwide, tuberculosis (TB) is a major public health problem and the rapid diagnosis and appropriate chemotherapy become the first priority and a serious challenge to improve TB treatment. In the objective of early TB diagnosis and rapid detection of Mycobacterium tuberculosis (MTB) in the clinical specimens, the utility of the Polymerase Chain Reaction (PCR) using the Insertion Sequence 6110 “IS6110" as target was compared to conventional methods. Methods: Out of 305 patients with different clinical mani drug failure and chronic cases were enrolled in this study and tested by mycobacteriological and PCR techniques for the investigation about the tubercle bacilli. Results: The results of the in house “IS6110" PCR sh specificity (98.0%), the positive and negative predictive values were 96.4 % and 95.3 % respectively. Conclusion: This study showed clearly that the PCR testing using the “IS6110" in the routine analysis is a potential tool for the rapid TB diagnosis, especially for critical cases and would be of great interest to help the clinician in the misdiagnosed critical cases by the traditional radiology. www.mjhid.org ISSN 2035-3006 with Conventional Methods for the in Morocco , Nada Bouklata, Sanae Jaouhari , Abdelaziz Benjouad, My Mustapha Ennaji . Mohammedia-Casablanca. . Laboratory of Virology, Hygiene & Tel: +212661748862/ 212662013772, Fax: 212523315353. E at no competing interests exist. 2, 4(1): e2012049, DOI: 10.4084/MJHID.2012.049 ), which permits unrestricted use, distribution, and reproduction in any medium,